Respirational activity of Chlorella fusca monitored by in vivo P-31 NMR |
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Authors: | J Sianoudis A C Küsel T Naujokat W Offermann A Mayer L H Grimme D Leibfritz |
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Institution: | (1) Fachbereich 1-Physik der Universität Bremen, D-2800 Bremen 33, Federal Republic of Germany;(2) Fachbereich 2-Biologie der Universität Bremen, D-2800 Bremen 33, Federal Republic of Germany;(3) Fachbereich 2-Chemie der Universität Bremen, D-2800 Bremen 33, Federal Republic of Germany |
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Abstract: | Energy metabolism during dark respiration of the green alga Chlorella fusca was investigated by 31P NMR spectroscopy. The kinetics of the transition from anaerobic to aerobic conditions (and vice versa) was followed with a temporal resolution of 16 s. This transition is accompanied by a shift of the cytoplasmic pH from 6.8 to 7.4, while the vacuolar pH remains constant. Simultaneously, an increase in the concentration of nucleoside-triphosphates and a decrease in the concentration of cytoplasmic orthophosphate take place, as well as the formation of mobile polyphosphates. The concentration of ATP and P
i reach steady-state levels within 30 s. Upon the reverse transition, from aerobic to anaerobic conditions, steady-state concentrations are obtained only after 3 min. |
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Keywords: | 31P NMR respiration polyphosphate Chlorella intracellular pH |
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