Similarities in protein synthesis and degradation in normal and dystrophic muscle cultures |
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Authors: | Barry A. Wolitzky Harold L. Segal Michael S. Hudecki |
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Affiliation: | Division of Cell and Molecular Biology, State University of New York at Buffalo, Buffalo, NY 14260, USA |
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Abstract: | Protein synthesis and degradation were compared in cultured muscle cells obtained from normal and dystrophic chick embryos under conditions where labeled amino acid reincorporation was not a complicating factor, where fibroblast contamination was minimized, and where the animals compared were as genetically similar as possible. Under these conditions both cell types exhibited a half-time of protein turnover of 34 h. Degradation in both was inhibited 21% by leupeptin (50 μg/ml), and both showed parallel increases in degradation rates under ‘step-down’ conditions. |
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