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Expression of Bacillus macerans cyclodextrin glucanotransferase gene in Saccharomyces cerevisiae |
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| Authors: | Soo-Wan Nam Hyun-Yi Park Jong-Hyun Kim Jin-Ho Seo Nam-Soo Han Byung-Woo Kim |
| Institution: | (1) Department of Microbiology, Dong-Eui University, Pusan, 614-714, Korea;(2) Department of Food Science & Technology and Research Center for New Bio-Materials in Agriculture, Seoul National University, Suwon, 441-744, Korea;(3) Department of Food Science & Technology, Chungbuk National University, Cheongju, 361-763, Korea |
| Abstract: | Cyclodextrin glucanotransferase (CGTase) gene of Bacillus macerans was subcloned down-stream of yeast ADH1 promoter and expressed in Saccharomyces cerevisiae. Most of the CGTase expressed was in the extracellular medium with a maximum activity of about 0.28 unit ml–1 after 48 h cultivation. The recombinant CGTase was secreted as an N-linked-glycosylated form and predominantly produced  -cyclodextrin from starch. |
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