A nematode demographics assay in transgenic roots reveals no significant impacts of the Rhg1locus LRR-Kinase on soybean cyst nematode resistance |
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Authors: | Sara Melito Adam L Heuberger David Cook Brian W Diers Ann E MacGuidwin Andrew F Bent |
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Affiliation: | (1) Department of Plant Pathology, University of Wisconsin – Madison, Madison, WI 53706, USA;(2) Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA |
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Abstract: | Background Soybean cyst nematode (Heterodera glycines, SCN) is the most economically damaging pathogen of soybean (Glycine max) in the U.S. The Rhg1 locus is repeatedly observed as the quantitative trait locus with the greatest impact on SCN resistance. The Glyma18g02680.1 gene at the Rhg1 locus that encodes an apparent leucine-rich repeat transmembrane receptor-kinase (LRR-kinase) has been proposed to be the SCN resistance gene, but its function has not been confirmed. Generation of fertile transgenic soybean lines is difficult but methods have been published that test SCN resistance in transgenic roots generated with Agrobacterium rhizogenes. |
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