| 家蚕基因特异性CAPs标记获得及其分子系统学应用 |
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| 引用本文： | 黄健华,苗雪霞,李木旺,张勇,赵卫国,黄勇平. 家蚕基因特异性CAPs标记获得及其分子系统学应用[J]. 遗传, 2005, 27(4): 584-588 |
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| 作者姓名： | 黄健华 苗雪霞 李木旺 张勇 赵卫国 黄勇平 |
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| 作者单位： | 中国科学院上海生命科学研究院植物生理生态研究所，上海 200032 |
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| 基金项目： | 上海市科委资助项目，中国科学院资助项目 |
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| 摘 要： | 选取家蚕attacin和alpha-amylase基因序列，设计特异性引物，在家蚕品系P50、C108和子一代 (F1) 中扩增。分别采用4种不同的限制性内切酶对扩增产物酶切，最后每个基因都获得了一个CAPs分子标记。依据所得的两个CAPs分子标记对12个品系的家蚕遗传多样性进行了初步研究，构建了其分子系统树。
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| 关 键 词： | 家蚕 CAPs标记 多态 酶切 分子系统树 |
| 文章编号： | 0253-9772(2005)04-0584-05 |
| 收稿时间： | 2004-08-10 |
| 修稿时间： | 2004-08-10 |
The Acquisition of Two Silkworm CAPs Markers and Their Use in Genetic Diversity and Phylogenetic Relationship |
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| HUANG Jian-Hua,MIAO Xue-Xia,LI Mu-Wang,HANG Yong,ZHAO Wei-guo,HUANG Yong-Ping. The Acquisition of Two Silkworm CAPs Markers and Their Use in Genetic Diversity and Phylogenetic Relationship[J]. Hereditas, 2005, 27(4): 584-588 |
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| Authors: | HUANG Jian-Hua MIAO Xue-Xia LI Mu-Wang HANG Yong ZHAO Wei-guo HUANG Yong-Ping |
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| Affiliation: | Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | Cleaved amplified polymorphic sequence (CAPs) markers are based on PCR amplification of known genes, cDNA sequences or RAPD sequences. The PCR products are digested by restriction enzymes, generating the simple type of data as heterozygotes and homozygotes. Here we designed primers based on silkworm attacin and alpha-amylase genes, then digested the PCR products in silkworm strains P50, C108 and their progeny F1 using 4 different restriction enzymes respectively. Furthermore, the genetic diversity and phylogenetic relationship of 12 silkworm strains were investigated using the obtained two CAPs markers. |
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| Keywords: | Bombyx mori (L.) CAPs polymorphism enzyme digestion molecular dendrogram |
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