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Antifreeze activity in the cerambycid beetle Rhagium inquisitor
Authors:E Kristiansen  S Pedersen  H Ramløv  K E Zachariassen
Institution:(1) Laboratory of Ecophysiology and Toxicology, Department of Zoology, Norwegian University of Science and Technology, N-7034 Trondheim, Norway e-mail: kaz@chembio.ntnu.no, Fax: +47-73-59-63-11, NO;(2) Department of Chemistry and Life Sciences, Roskilde University, Postbox 260, DK-4000 Roskilde, Denmark, DK
Abstract:The present study revealed that hibernating freeze-avoiding Rhagium inquisitor beetles have thermal hysteresis antifreeze agents in the intracellular fluid as well as in the intestinal fluid and the haemolymph. The antifreeze activity in all three compartments increased with diminishing size of the seeding ice crystal, suggesting that all three compartments are well protected against spontaneous ice nucleation at low sub-zero temperatures. Accepted: 4 October 1998
Keywords:Coleoptera  Cerambycidae  Antifreeze proteins  Thermal hysteresis  Body fluid compartments
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