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A rapid method for measuring the production of human migration inhibitory factor (MIF)
Authors:E A Lipsmeyer
Affiliation:Department of Medicine, University of Arkansas School of Medicine, Little Rock, Arkansas 72201 U.S.A.
Abstract:A rapid method for assaying Migration Inhibitory Factor (MIF) prepared from human peripheral venous blood is described. Using increased concentrations of human peripheral lymphocytes, MIF could be demonstrated in supernatant at the end of a 24 hr culture period. Both tuberculin and histoplasmin sensitivity were investigated. In persons with normal cell-mediated immunity, there was good correlation between skin test sensitivity and MIF production by this method.
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