Bacterial, archaeal, and eukaryal diversity in the intestines of Korean people |
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Authors: | Young-Do Nam Ho-Won Chang Kyoung-Ho Kim Seong Woon Roh Min-Soo Kim Mi-Ja Jung Si-Woo Lee Jong-Yeol Kim Jung-Hoon Yoon Jin-Woo Bae |
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Institution: | (1) Biological Resources Center, KRIBB, Daejeon, 305-806, Republic of Korea;(2) University of Science and Technology, Daejeon, 305-333, Republic of Korea;(3) Korea Institute of Oriental Medicine, Daejeon, 305-811, Republic of Korea;(4) Environmental Biotechnology National Core Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea |
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Abstract: | The bacterial, archaeal, and eukaryal diversity in fecal samples from ten Koreans were analyzed and compared by using the
PCR-fingerprinting method, denaturing gradient gel electrophoresis (DGGE). The bacteria all belonged to the Firmicutes and Bacteroidetes phyla, which were known to be the dominant bacterial species in the human intestine. Most of the archaeal sequences belonged
to the methane-producing archaea but several halophilic archarea-related sequences were also detected unexpectedly. While
a small number of eukaryal sequences were also detected upon DGGE analysis, these sequences were related to fungi and stramenopiles
(Blastocystis hominis). With regard to the bacterial and archaeal DGGE analysis, all ten samples had one and two prominent bands, respectively,
but many individual-specific bands were also observed. However, only five of the ten samples had small eukaryal DGGE bands
and none of these bands was observed in all five samples. Unweighted pair group method and arithmetic averages clustering
algorithm (UPGMA) clustering analysis revealed that the archaeal and bacterial communities in the ten samples had relatively
higher relatedness (the average Dice coefficient values were 68.9 and 59.2% for archaea and bacteria, respectively) but the
eukaryal community showed low relatedness (39.6%). |
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Keywords: | human intestinal microbes Bacteria Archaea Eukaryote DGGE UPGMA |
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