在大肠杆菌中分别表达汉滩病毒素膜糖蛋白G1和G2

利用PCR方法扩增了汉滩病毒76－118株囊膜糖蛋白G1和G2的编码区基因，并将PCR产物克隆到T-载体中，用限制性内切酶将G1和G2的编码区基因切下，并克隆到表达载体pBV220中构建G1和G2的表达质粒。诱导表达后在SDS－PAGE凝胶中未见表达产物带，表达的G1和G2能与部分抗G1和G2的单克隆抗体发生反应，但用Western－blot方法不能检测到表达产物。用表达的G1和G2免疫小白鼠能刺激小白鼠产生特异性抗汉摊病毒的抗体，间接免疫荧光抗体的滴度可分别达到1:160和1：320。

Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E. coli

The expression vector plasmid pBV220 was used to express the envelope glycoproteins G1 and G2 of Hantaan virus in E. coli. First, the coding frame genes of G1 and G2 were amplified by PCR, and cloned into th6 PCR product cloning T - vector, then they were cut off using restriction endonucleases from the T - vector cloning plasmids. Last, the expression plasmids of G1 and G2 were constructed by ligating the conding frames of G1 and G2 with expression vector plasmid. The expressed proteins could not be seen on SDS - PAGE gel. They could be reacted with a part of monoclonal antibodies against Hantaan virus G1 and G2, but not be tested in Western - blot. Mice were immunized with expressed G1 and G2, antibody against Hantaan virus could be tested in the sera of the immunized mice, the IFA(Indirect Immunofluorescence Assay)antibody titers were 1:160 and 1 :320, respectively.