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Protoplasts as tools for plant genome modifications |
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| Authors: | Jerzy Paszkowski Alex Peterhans Henriette Schlüpmann Christoph Basse Edouard G Lebel Jean Masson |
| Institution: | Inst. of Plant Sciences, Swiss Federal Inst. of Technology, ETH-Zürich, Universitätstr. 2, CH-8092 Zürich, Switzerland;Lab. de Biologie Cellulaire INRA, Centre de Versailles, Route de Saint-Cyr, F-78026 Versailles, France. |
| Abstract: | We present here the application of protoplast technology in the selection and recovery of rare, spontaneous plant genome alterations. Using protoplasts as a cell cloning system allowed the detection and molecular characterization of intrachromosomal recombination events between genomic repeats. The mechanism, frequencies and the induction of intrachromosomal recombination are discussed as well as its application for genome mutagenesis. |
| Keywords: | Cell cloning homologous recombination intrachromosomal recombination plant protoplasts repeated DNA |