| 融合gp67信号肽的慈菇蛋白酶抑制剂基因在蚕体内表达 |
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| 引用本文： | 季平,吴祥甫.融合gp67信号肽的慈菇蛋白酶抑制剂基因在蚕体内表达[J].生物工程学报,1999,15(3):310-314. |
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| 作者姓名： | 季平 吴祥甫 |
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| 作者单位： | 中国农业科学院蚕业研究所农业部家蚕生物技术重点开放实验室，中国科学院上海生物化学研究所 |
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| 基金项目： | 国家高技术研究发展计划项目 |
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| 摘 要： | 用ＡｃＭＮＰＶ的ｇｐ６７信号肽与慈菇蛋白酶抑制剂基因（ＡＰＩ２）融合，并整合到昆虫病毒表达载体ＢｍＢａｃＰＡＫ６中，受多角体蛋白基因启动子控制。慈菇蛋白酶抑制剂在蚕体内成功地得到了高效表达。比较了ｇｐ６７信号肽和慈菇蛋白酶抑制剂信号肽在昆虫表达系统中对表达产物的影响，发现表达产物都能分泌到血淋巴中，表达量很相似，但这两种信号肽在表达过程中都没有被切除，且不同信号肽对表达产物的生物活性有很大影响。
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| 关 键 词： | 家蚕核型多角体病毒，蛋白酶抑制剂，信号肽，家蚕幼虫 |
Expression of the Arrowhead Proteinase Inhibitor Gene Fused gp67 Signal Sequence in the
Silkworm Larvae |
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| Ji Ping,Xiao Qingli,He Jialu,Lu Hongsheng,Wu Xiangfu.Expression of the Arrowhead Proteinase Inhibitor Gene Fused gp67 Signal Sequence in the
Silkworm Larvae[J].Chinese Journal of Biotechnology,1999,15(3):310-314. |
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| Authors: | Ji Ping Xiao Qingli He Jialu Lu Hongsheng Wu Xiangfu |
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| Institution: | Ji Ping 1 Xiao Qingli 1 He Jialu 2 Lu Hongsheng 1 Wu Xiangfu 2
1 |
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| Abstract: | The arrowhead proteinase inhibitor API 2
gene derived from API 4 gene by PCR. API 2 gene was fused to gp67 signal sequence,and
recombinanted into the lineared Bm BacPAK6 DNA digested with Cvn I.The recombinant virus
CrBK gp API 2 was selected. API 2 gene was successfully expressed in silkworm
larvae.Compared the function of gp67 and API signal sequence in insect expression
system,found that the two signal peptides didn't be cleaved.The amount of secreted protein is
almost same in the insect cell system.But it has been observed that different signal peptides
greatly affected the bioactivity of the expressed gene products. |
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| Keywords: | Bombyx mori nuclear polyhedrosis virus (BmNPV) proteinase inhibitor signal peptide sequence silkworm larvae |
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