贴壁培养方式中昆虫细胞的生长限制性因素研究

以昆虫细胞为宿主生产病毒杀虫剂或进行基因工程产品的开发，是动物细胞培养领域十分有吸引力的研究方向。近年来，昆虫细胞的体外培养尽管在培养条件的优化⑴、培养基的开发⑵以及工艺癍程的设计⑶等诸多方面取得了令人鼓舞的进展，但由于对影响细胞生长及代谢的各类限制性因素尚缺乏全面系统的了解，因而目前的技术水平还远不能设计出优化的培养系统以满足产业化的需要。众所周知，细胞的生长和产物的形成是多种环境因素和细胞内复杂代谢反应的综合结果，因此。对生长限制性因素的考察也应有全局观念，即：在重视生化环境(外因)对细胞培养过程影响的同时，也不能忽视细胞株自身性能(内因)对最终培养结果的影响。以往的研究〔4.5〕大多仅就个别营养限制性因素进行孤立地探讨，缺乏从内外因两方面对昆虫细胞培养过程的认识。最近，以微载体技术或堆积床技术贴壁培养昆虫细胞业已证明具有很大的发展前途〔6.7〕。为此．本文以贴壁培养的秋黏虫细胞(IPLB-Sf2l—AE)和粉蚊夜蛾细胞(BTI—Tn一5Bl-4)为研究对象，在考察环境限制性因素的同时，亦从细胞自身特点出发考察了不同细胞株的生长及代谢性能。研究结果可望为贴壁培养技术的深入应用以及培养过程的合理设计提供参考。

Identification of Growth limiting Factors for Insect Cells Grown in Stationary Cultures

An investigation was undertaken into the growth properties of two different insect cell lines grown in stationary cultures.The environmental factors which could affect the growth of attached cells were identified and a significant increase in density of attached cells were obtained by optimization of several environmental factors.In addition to the identification of environmental factors,the study also went deep into the growth characteristics inherent in different cell lines.It was shown that BTI Tn 5B1 4 cells were stricter with culture conditions as compared with IPLB Sf21 AE cells.Under identical culture conditions,BTI Tn 5B1 4 cells had both lower growth rate and shorter exponential growth phase than IPLB Sf21 AE cells.Further analysis of metabolic rates revealed that higher glucose utilization rate but lower yield factor was typical of BTI Tn 5B1 4 cells.