白魔芋和花魔芋葡甘露聚糖研究

从白魔芋和花魔芋的块茎中分离纯化出两种魔芋葡甘露聚糖(Konjac Glucomannan,简称KGM),分别名为白魔芋葡甘露聚糖(aKGM)和花魔芋葡甘露聚糖(rKGM)。通过超离心、玻璃纤维纸电泳和凝胶过沪证明aKGM和rKGM都是均一的多糖。这两种多糖都由甘露糖(M)和葡萄糖(G)组成,其克分子比G/M:aKGM为1:1.69,rKGM为1:1.60,分子量前者为8.09×10~5,后者为7.37×10~5。酶水解实验和红外光谱分析说明aKGM和rKGM都是由甘露糖和葡萄糖以β-1,4-糖苷键连接的杂多糖;有O-乙酰基的特征吸收峰,说明在某些糖残基上可能有乙酰基团。

STUDIES of GLUCOMANNANS FROM AMORPHOPHALLUS ALBUS AND AMORP HO PHALLUS RIVIERI

Two kinds of konjac glucomannans (KGM) ,named aKGM and rKGM were isolated from the tubers of Amorphophallus albus and Amorphophallus rivieri respectively. They were homogeneous in ultracentrifugal analysis, glass-fiber electrophor-esis and gel filtration.Both of them were composed of D-glucose(G)aud D-manno-se(M).The molar ratio of G aud M iu aKGM was 1:1.69, and that iu rKGM was 1:1.6. Their molecular weights were estimated to be 8 .0 ×910s and 7.37 × 10s respectively .Enzymatic hydrolysis experiment aud infra-red absorptiou spectrum analysis showed that the D-glucose aud D-mannose fesidues of both KGMs were joined together by β-1,4-linkages. It seemed that some sugar residues had o-acetyl group.