Purification of almond emulsin alpha-L-fucosidase I by affinity chromatography. |
| |
Authors: | H Yoshima S Takasaki S Ito-Mega A Kobata |
| |
Institution: | Department of Biochemistry, Kobe University School of Medicine, Ikutaku, Kobe 650, Japan |
| |
Abstract: | An affinity chromatographic method to purify α-l-fucosidase I from almond emulsin was developed. A derivative of lacto-N-fucopentaose II, ?-aminocaproyl-lacto-N-fucopentaosylamine, was coupled to sepharose 4B and packed in a column. By adopting this column for affinity chromatography, the enzyme was purified a hundredfold. The enzyme preparation was free from any other exoglycosidases which act on natural substrates. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|