Somatic embryogenesis in Eucalyptus globulus |
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| Authors: | Nugent Greg Chandler Stephen F Whiteman Phil Stevenson Trevor W |
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| Institution: | (1) Department Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, GPO Box 2476V, 3001, Melbourne, 3001, Australia;(2) Florigene Pty. Ltd., 16 Gipps Street, Collingwood, 3066, Australia;(3) Australian Paper Plantations Pty. Ltd., P.O. Box 220, Morwell, 3840, Australia;(4) Present address: Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland |
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| Abstract: | Somatic embryos were obtained from 1% of cotyledon pieces and hypocotyls of mature embryos of Eucalyptus globulus Labill. cultured on media containing a high concentration of picloram or IBA. 2,4-D and other synthetic auxins did not yield somatic embryos or embryogenic callus. Somatic embryos arose indirectly via callus, being visible after four months, and directly, where little callus or adventitious root initiation occurred. Somatic embryos, formed directly from explants, were visible within five weeks. Various structural abnormalities of somatic embryos were observed, especially after induction on media containing picloram. Only two out of fifteen somatic embryos showed hypocotyl and radical elongation, but plantlets did not develop further. |
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