Novel ion channels in the protists, Mougeotia and Saprolegnia, using sub-gigaseals. |
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Authors: | R R Lew A Garrill L Covic I B Heath B S Serlin |
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Institution: | York University, Department of Biology, Ont., Canada. |
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Abstract: | Protoplasts of the filamentous alga, Mougeotia, and the filamentous fungal oomycete, Saprolegnia ferax, exhibit two K+ ion channels (2–6 pA) using the patch-clamp technique when the seals are less than 1 GΩ (about 100 MΩ). The membrane potential of the protoplasts was near 0 mV as measured intracellularly with double-barreled micropipettes; thus, inward K+ flux is due solely to concentration differences. Although conductances are in the range expected for K+ channels, the activity at 0 mV is not seen in other organisms under gigaseal conditions. This paper draws attention to the usefulness of this subsidiary patch-clamp technique and the novel characteristics of ion channels in Mougeotia and Saprolegnia. |
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Keywords: | Mougeotia Saprolegnia Extracellular patch clamp Calcium-activated potassium channel Stretch-activated channel Sub-gigaseal |
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