细菌L型的厌氧诱导和培养

厌氧条件下以羧卡青霉素诱导金黄色葡萄球菌、大肠杆菌和蜡样芽胞杆菌形成Ｌ型，观察细菌Ｌ型在厌氧条件下的形成、形态、生长及时渗透压的敏感性等特性。结果表明：蜡样芽胞杆菌在厌氧条件下不能形成Ｌ型或其Ｌ型在厌氧条件下亦不能返祖。金黄色葡萄球菌和大肠杆菌在厌氧条件下虽能诱生Ｌ型，但形成丝状体的构成Ｌ型菌落难以传代培养，厌氧培养未见Ｌ型圆球体和典型Ｌ型油煎蛋样菌落。金黄色葡萄球菌Ｌ型在含１％～１０％ＮａＣｌ的Ｌ型培养基上可生长形成Ｌ型菌落或非菌落形式存在的Ｌ型巨形体；大肠杆菌和蜡样芽胞杆菌的Ｌ型在含２％～６％ＮａＣｌ的Ｌ型培养基上可生长形成Ｌ型菌落或非菌落形式存在的Ｌ型巨形体。涂片染色或返祖试验证实细菌Ｌ型在含０．５％ＮａＣｌ的Ｌ型培养基或常规细菌学培养基上亦可生存。非菌落性Ｌ型巨形体和丝形体是细菌Ｌ型在琼脂培养基上广泛的存在形式。

INDUCEMENT AND CULTURE OF BACTERIALL-FORMS IN ANAEROBIC ENVIRONMENT

L-forms of.aureus.E.coli and B. cereus were induced by carbenicillin anaerobly and thd morphology,growth and osmotic pressure susceptibility of thd L-form were studied. It was shown that L-forms of B. cereus neither form nor grow in anaerobic enriroment. The L-form of s.aureus could grow and form the L-form colonies or non-colonic L-spheroplasts on L-form media with 1%-10%NaCl.L-form of E.coli and B. cereus could form the L-form colonies or non-colonic L-spheroplasts on L-form media with 2%-6%NaCl in areobic cultures. Presence of bacterial L-forms on the L-form medium with 0.5%NaCl or routine bacteriologic media would be confirmed by stain or reversion.The noncolonic L-spheroplasts and filaments of bacterial L-form were common sight on agar media.