Transposon mediated transgenesis in a marine invertebrate chordate: <Emphasis Type="Italic">Ciona intestinalis</Emphasis> |
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| Authors: | Yasunori Sasakura Yuichi Oogai Terumi Matsuoka Nori Satoh Satoko Awazu |
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| Institution: | Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan. sasakura@kurofune.shimoda.tsukuba.ac.jp |
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| Abstract: | Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis. |
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