Factors affecting the immunogenicity of tetanus toxin fragment C expressed in Lactococcus lactis |
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Authors: | Pamela M Norton Henry WG Brown Jeremy M Wells Angela M Macpherson Peter W Wilson Richard WF Le Page |
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Institution: | Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK |
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Abstract: | Abstract The relative immunogenicity of tetanus toxin fragment C (TTFC) has been determined in three different strains of inbred mice when expressed in Lactococcus lactis as a membrane-anchored protein (strain UCP1054), as an intracellular protein (strain UCP1050), or as a secreted protein which is partly retained within the cell wall (strain UCP1052). Protection against toxin challenge (20 × LD50) could be obtained without the induction of anti-lactococcal antibodies. When compared in terms of the dose of expressed tetanus toxin fragment C required to elicit protection against lethal challenge the membrane-anchored form was significantly (10–20 fold) more immunogenic than the alternative forms of the protein. |
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Keywords: | Lactococcus lactis Targeting antigen Tetanus toxin fragment C Bacterial vaccine vector Vaccine |
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