Isolation and characterization of microsatellite loci in the endangered freshwater fish <Emphasis Type="Italic">Pararasbora moltrechti</Emphasis> (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA) |
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Authors: | Hung-Du Lin Teh-Wang Lee Fang-Jiao Lin Chung-Jian Lin Tzen-Yuh Chiang |
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Institution: | (1) Department of Life Sciences, National Cheng Kung University, Tainan, 701, Taiwan;(2) Taiwan Endemic Species Research Institute, ChiChi, Nantou, 552, Taiwan |
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Abstract: | Pararasbora moltrechti (Cyprinidae) is an endangered freshwater fish with a scattered distribution in the central Taiwan. In order to undertake
a conservation program for this species in Taiwan, it is essential to evaluate its genetic diversity and population structure.
For this purpose, we described the development of eight microsatellite loci in P. moltrechti for genetic studies. These new molecular markers were tested in 36 individuals of the rare species. The number of alleles
per locus ranged from 8 to 14. As the expected heterozygosity varied from 0.770 to 0.932, all microsatellite loci, however,
were significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency.
Hung-Du Lin and Teh-Wang Lee contributed equally. |
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Keywords: | Microsatellite PIMA RAPD-PCR enrichment Pararasbora moltrechti |
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