Adventitious shoot formation from leaf explants of Rhododendron |
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| Authors: | Giovanni Iapichino Steve MeCullech Tony H. H. Chen |
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| Affiliation: | (1) Department of Horticulture, Oregon State University, Cordley Hall 2042, 97331-2911 Corvallis, OR, USA;(2) Present address: Istituto di Orticoltura e Floricoltura, Universitá di Palermo, 90100, Italy;(3) Present address: Briggs Nursery, Inc., 98501 Olympia, WA, USA |
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| Abstract: | A protocol for high frequency adventitious shoot regeneration adventitious shoot regeneration from leaf explants of Rhododendron spp. has been developed. The highest percentage of regeneration and the greatest number of shoots were obtained when leaf explants were cultured on Anderson's medium containing 4.9  M IBA and 73.8 M 2iP. Genotypic variation was observed for adventitious shoot regeneration potential among the seven cultivars tested. Regeneration frequencies ranged from 0 to 96%.  Lodestar had the highest rate of regeneration after 3 months of culture with 96% shoot regeneration and an average of 14 shoots per explant. Regenerated shoots were rooted in soil in about 2 months. This protocol should be useful in applying gene transfer techniques to Rhododendron improvement.Abbreviations IAA 1-H-indole-3-acetic acid - NAA 1-naphthaleneacetic acid - IBA 1-H-indole-3-butyric acid - 2,4-d 2,4-dichlorophenoxyacetic acid - BA 6-benzyladenine - 2iP N-(3-methyl-2-butenyl)-1-H-purine-6-amine |
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| Keywords: | plant growth regulators shoot regeneration tissue culture |
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