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Modulation of smooth muscle actomyosin ATPase by thin filament associated proteins
Authors:K Y Horiuchi  H Miyata  S Chacko
Affiliation:1. Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23507, USA;2. Department of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL, USA;3. Department of Biological Science, Florida State University, Tallahassee, FL, USA;1. Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, Viale Morgagni 63, 50134 Florence, Italy;2. Bioengineering, University of Washington, Seattle, WA 98109, USA;3. Medicine, Division of Cardiology, University of Washington, Seattle, WA 98195, USA;4. Rehabilitation Medicine, University of Washington, Seattle, WA 98109, USA;5. Pathology, University of Washington, Seattle, WA 98109, USA;6. Medicine, Division of Cardiology, University of Colorado, Denver, CO 80262, USA;7. Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98109, USA;8. Center for Cardiovascular Biology, University of Washington, Seattle, WA 98109, USA;1. Department of Physics, Umeå University, Umeå, Sweden;2. Umeå Centre for Microbial Research (UCMR), Umeå, Sweden;3. Department of Paraclinical Sciences, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), Ås, Norway;1. Department of Physiology & Biophysics, Boston University Chobanian & Avedisian School of Medicine, Boston, MA 02118, USA;2. Department of Chemistry, The College of New Jersey, Ewing, NJ 08628, USA;3. Department of Physics, Umeå University, Umeå, Sweden;4. Umeå Centre for Microbial Research (UCMR), Umeå University, Umeå, Sweden
Abstract:Caldesmon binds equally to both gizzard actin and actin containing stoichiometric amounts of bound tropomyosin. The binding of caldesmon to actin inhibits the actin-activation of the Mg-ATPase activity of phosphorylated myosin only when the actin contains bound tropomyosin. The reversal of this inhibition requires Ca2+-calmodulin; but it occurs without complete release of bound caldesmon. Although phosphorylation of the caldesmon occurs during the ATPase assay, a direct correlation between caldesmon phosphorylation and the release of the inhibited actomyosin ATPase is not consistently observed.
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