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BisEDT and RIP act in synergy to prevent graft infections by resistant staphylococci |
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| Authors: | Domenico Philip Gurzenda Ellen Giacometti Andrea Cirioni Oscar Ghiselli Roberto Orlando Fiorenza Korem Moshe Saba Vittorio Scalise Giorgio Balaban Naomi |
| Affiliation: | aCardio Pulmonary Research Institute, Winthrop-University Hospital, SUNY Stony Brook School of Medicine, Mineola 11501, New York, NY, USA bInstitute of Infectious Diseases and Public Health, Università Politecnica delle Marche, Ancona, Italy cDepartment of General Surgery, I.N.R.C.A. I.R.R.C.S., Università Politecnica delle Marche, Ancona, Italy dResearch Department, Biotechnology Centre, I.N.R.C.A. I.R.R.C.S Università Politecnica delle Marche, Ancona, Italy eDepartment of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel fDepartment of Biomedical Sciences, Tufts University School of Veterinary Medicine, North Grafton, MA, USA |
| Abstract: | Staphylococci are a major cause of infections associated with indwelling medical devices. Biofilm formation on these devices adds to the antibiotic resistance seen among clinical isolates. RNAIII-inhibiting peptide (RIP) is a heptapeptide that inhibits staphylococcal pathogenesis, including biofilm formation, by obstructing quorum sensing mechanisms. Bismuth ethanedithiol (BisEDT) also prevents biofilm formation at subinhibitory concentrations. RIP and BisEDT were combined to prevent infections in a rat graft model, using antibiotic sensitive and resistant strains of Staphylococcus aureus and Staphylococcus epidermidis. BisEDT, RIP, or rifampin, or their combinations reduced the graft associated bacterial load over seven days. BisEDT–RIP was the best combination, reducing bacterial load to undetectable levels. BisEDT–RIP may prove useful for coating medical devices to prevent staphylococcal infections. |
| Keywords: | Bismuth thiols Staphylococci Antimicrobial peptides RNAIII inhibiting peptide Biofilms |
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