微生物分子生态学技术及其在环境污染研究中的应用

较为系统地概述了核酸探针检测技术、利用引物的PCR技术、DNA序列分析技术和电泳分离及显示技术在国内外的研究进展，并探讨了这些技术在环境污染研究中的应用及其方向。结果表明，这些被认为是重要的微生物分子生态学技术，在探索微生物与污染环境之间的相互关系中发挥了重要作用。促进了污染环境的微生物遗传适应进化机制的研究，污染物的微生物降解有关基因的定位及微生物工程菌的构建等方面的工作，从而推进了污染环境微生物修复的分子生态学的发展。

Molecular-ecological technology of microorganisms and its application to research on environmental pollution.

Nucleicacid probe detection,PCR technique using primer,DNA sequential analysis,and electrophoresis separation and display were summarized and the application of these techniques to research on environmental pollution and future developing directions were discussed.It was pointed out that molecular-ecological technology of microorganisms are playing an important role in studying on relationships between microorganisms and contaminated environment.There are some important advances such as genetic adaptation and evolutionary mechanisms of microorganisms in contaminated environment,the positioning of pollutant-degrading genes of microorganisms and the construction of microbiological engineering bacteria,thus promoting the development of molecular ecology for bioremediation of contaminated environment.