Studies in the enzymology of glutathione metabolism in human erythrocytes |
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| Authors: | R C Jackson |
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| Institution: | Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, London, S.W. 3 |
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| Abstract: | Spectrophotometric assay methods are described for glutathione synthetase, gamma-glutamylcysteine synthetase and gamma-glutamyl transpeptidase of erythrocytes. The contents of these enzymes in normal human erythrocytes are reported. Erythrocyte glutathione synthetase is inhibited by ADP; this inhibition is competitive with respect to ATP. gamma-Glutamylcysteine synthetase is subject to feedback inhibition by GSH, and is also inhibited by NADH, and to a lesser extent by NAD(+) and NADPH. This enzyme is irreversibly inactivated by cysteamine. |
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