| 黑胸散白蚁肠道共生锐滴虫目鞭毛虫的多样性分析与原位杂交鉴定 |
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| 引用本文： | 陈文,石玉,彭建新,洪华珠,杨红. 黑胸散白蚁肠道共生锐滴虫目鞭毛虫的多样性分析与原位杂交鉴定[J]. 生态学报, 2011, 31(18): 5332-5340 |
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| 作者姓名： | 陈文 石玉 彭建新 洪华珠 杨红 |
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| 作者单位： | 1. 遗传调控与整合生物学湖北省重点实验室,华中师范大学生命科学学院,武汉,430079
2. 遗传调控与整合生物学湖北省重点实验室,华中师范大学生命科学学院,武汉,430079;农药与化学生物学教育部重点实验室,华中师范大学,武汉,430079 |
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| 基金项目： | 国家自然科学基金资助项目(30570034) |
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| 摘 要： | 低等木食性白蚁肠道内的鞭毛虫在纤维素降解过程中扮演着重要的角色。黑胸散白蚁Reticulitermes chinensis Snyder是一种广泛分布于我国的低等木食性白蚁,然而目前对于其肠道内的共生鞭毛虫却鲜见报道。采用锐滴虫目18S rDNA特异引物扩增鞭毛虫18S rRNA 基因并构建文库,对得到的基因进行系统发育多样性分析。针对得到的序列设计特异性的荧光探针,用荧光原位杂交技术对锐滴虫目鞭毛虫进行了鉴定。从黑胸散白蚁肠道得到11个锐滴虫目鞭毛虫18S rDNA序列,它们之间的相似性为86.9%-99.3%。系统发育分析表明,锐滴虫目鞭毛虫主要属于Dinenympha和Pyrsonympha两个属。应用荧光原位杂交技术鉴定出了Dinenympha parva、Dinenympha exilis和Pyrsonympha sp.三种锐滴虫。研究表明,在黑胸散白蚁肠道共生的锐滴虫为Dinenympha和Pyrsonympha属的鞭毛虫。
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| 关 键 词： | 黑胸散白蚁 锐滴虫目鞭毛虫 系统发育多样性 荧光原位杂交 |
| 收稿时间： | 2011-02-12 |
| 修稿时间： | 2011-08-01 |
Phylogenetic diversity analysis and in situ hybridization of symbiotic Oxymonad flagellates in the hindgut of Reticulitermes chinensis Snyder |
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| CHEN Wen,SHI Yu,PENG Jianxin,HONG Huazhu and YANG Hong. Phylogenetic diversity analysis and in situ hybridization of symbiotic Oxymonad flagellates in the hindgut of Reticulitermes chinensis Snyder[J]. Acta Ecologica Sinica, 2011, 31(18): 5332-5340 |
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| Authors: | CHEN Wen SHI Yu PENG Jianxin HONG Huazhu YANG Hong |
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| Affiliation: | Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China;Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China;Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China;Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China |
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| Abstract: | |
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| Keywords: | Reticulitermes chinensis Snyder oxymonad flagellates phylogenetic diversity fluorescence in situ hybridization |
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