褐菖鲉肝CYP 1A作为生物标志物监测厦门海域石油污染状况

以褐菖鲉为实验鱼类,以鱼肝微粒体CYP1A 生物标志物(EROD活性和CYP1A蛋白表达量)为指标,在厦门海域开展了两次野外监测实验,研究EROD活性和CYP1A蛋白表达量的变化,以及它们与海水和沉积物中石油类和重金属含量之间的相关性。结果表明,在现场属于一类海水的石油类浓度(0.0121-0.0242 mg/L)条件下,石油类就能够显著诱导褐菖鲉肝EROD活性和CYP1A蛋白表达量,鱼肝EROD活性和CYP1A蛋白表达量与海水中石油类含量均呈现极显著正相关,CYP1A蛋白表达量比EROD活性较为敏感和稳定。此外,在监测实验中,尚未发现这两种生物标志物受所监测海区的海水和沉积物重金属含量的影响。因此,利用褐菖鲉肝微粒体EROD活性和CYP1A蛋白表达量作为生物标志物监测海洋石油类及其PAHs污染是可行的,在海洋环境石油类污染监测及其生化效应评价中具有重要的应用价值。而且,把这两种生物标志物结合起来加以研究并推广应用将更有意义。

A study on application of hepatic microsomal CYP1A biomarkers from Sebastiscus marmoratus to monitoring oil pollution in Xiamen waters

Two field experiments were performed for monitoring oil pollution in Xiamen waters by using 2 hepatic CYP1A biomarkers, microsomal EROD activity and CYP1A protein expression level from Sebastiscus marmoratus. The inducibility of EROD activity and CYP1A protein expression level and the correlation of CYP1A biomarkers with the concentrations of oil and heavy metals in the seawater and sediment were studied, respectively. The results showed that both the hepatic EROD activity and CYP1A protein expression were highly induced by oil concentrations (0.0121-0.0242 mg/L) in the waters belonging to the first class of seawater quality. Both the biomarkers showed great significant positive correlations with the oil concentrations of the seawater, and CYP1A protein expression was more sensitive and more stable than EROD activity to some content. Additionally, it was not found that both biomarkers were affected by the contents of heavy metals in the seawater and sediment in the present study. Thus, the results indicate that using hepatic EROD activity and CYP1A protein expression of Sebastiscus marmoratus as biomarkers is feasible for monitoring oil and its PAH pollution in marine environment and that both the biomarkers are important for monitoring the organic pollution in marine environment and assessing the biochemical effects of the pollution. Moreover, it is better to combine these two biomarkers in an indicator system for further study and application.