Passive Ca overload in H9c2 cardiac myoblasts: Assessment of cellular damage and cytosolic Ca transients |
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Authors: | Fernando Soler Antonio Lax M Carmen Asensio Domingo Pascual-Figal Francisco Fernández-Belda |
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Institution: | aDepartamento de Bioquímica y Biología Molecular A, Universidad de Murcia, Campus de Espinardo, 30071 Murcia, Spain;bDepartamento de Medicina Interna (Cardiología), Facultad de Medicina, Universidad de Murcia, Campus de El Palmar, 30120 Murcia, Spain |
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Abstract: | Increase of resting Ca2+ levels and amplitude of vasopressin-induced Ca2+ transients were observed when cells in serum-free medium were exposed to 5 mM Ca2+ for 2 h. Small effect on cell viability was also observed. A rapid cytotoxic effect was developed in the presence of 10 mM Ca2+ and absence of serum. However, cells exposed to 10 mM Ca2+ in the presence of serum were protected from damage for at least 2 days. Resting Ca2+ levels and cytosolic Ca2+ transients in serum-containing medium with 10 mM Ca2+ displayed lower increases and a tendency to recover control values. When serum was absent, cells preincubated with 10 mM Ca2+ were more sensitive to thapsigargin-induced damage than cells preincubated with lower Ca2+. The sensitivity was similar when serum was present. Tolerance to high Ca2+ in the presence of serum was linked to potentiation of the mitochondrial Ca2+ entry to decrease the sarcoplasmic reticulum Ca2+ overload. |
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Keywords: | Sarcoplasmic reticulum Thapsigargin Ca2+ overload Ca2+ transient Cell damage H9c2 cell |
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