Identification of molecular markers associated with mite resistance in coconut (Cocos nucifera L.). |
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Authors: | K V Shalini S Manjunatha P Lebrun A Berger L Baudouin N Pirany R M Ranganath D Theertha Prasad |
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Institution: | Plant Molecular Biology Laboratory, Department of Biotechnology, University of Agricultural Sciences, GKVK, Bangalore 560065, India. |
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Abstract: | Coconut mite (Aceria guerreronis 'Keifer') has become a major threat to Indian coconut (Co?cos nucifera L.) cultivators and the processing industry. Chemical and biological control measures have proved to be costly, ineffective, and ecologically undesirable. Planting mite-resistant coconut cultivars is the most effective method of preventing yield loss and should form a major component of any integrated pest management stratagem. Coconut genotypes, and mite-resistant and -susceptible accessions were collected from different parts of South India. Thirty-two simple sequence repeat (SSR) and 7 RAPD primers were used for molecular analyses. In single-marker analysis, 9 SSR and 4 RAPD markers associated with mite resistance were identified. In stepwise multiple regression analysis of SSRs, a combination of 6 markers showed 100% association with mite infestation. Stepwise multiple regression analysis for RAPD data revealed that a combination of 3 markers accounted for 83.86% of mite resistance in the selected materials. Combined stepwise multiple regression analysis of RAPD and SSR data showed that a combination of 5 markers explained 100% of the association with mite resistance in coconut. Markers associated with mite resistance are important in coconut breeding programs and will facilitate the selection of mite-resistant plants at an early stage as well as mother plants for breeding programs. |
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