Immobilization of Aspergillus ficuum phytase: product characterization of the bioreactor |
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Authors: | A H Ullah B Q Phillippy |
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Affiliation: | Southern Regional Research Laboratory ARS, USDA, New Orleans, Louisiana 70124. |
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Abstract: | Aspergillus ficuum phytase was covalently immobilized on Fractogel TSK HW-75 containing 2-oxy-l-alkylpyridinium salts. A packed-bed bioreactor was constructed with the immobilized phytase. An HPLC ion-exchange method was used to analyze the enzymatic products of the bioreactor. Immobilized fungal phytase was able to hydrolyze myo-inositol Hexa-, penta-, tetra-, tri-, and diphosphates. When the substrate solution was recirculated for 5 hr in the bioreactor about 50% inorganic orthophosphate was released and myo-inositol-diphosphate and mono-phosphate were the only remaining products. |
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