Effects of boron deficiency in cell suspension cultures of Populus alba L. |
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Authors: | Koichi Kakegawa Tadashi Ishii Toshiro Matsunaga |
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Institution: | (1) Department of Forest Chemistry, Forestry and Forest Products Research Institute, Matsunosato 1, Tukuba, Ibaraki, 305-8687, Japan;(2) Department of Agro-Environmental Research, National Agricultural Research Center for Kyushu Okinawa Region, Nishigoshi, Kumamoto, 861-1192, Japan |
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Abstract: | Cell suspension cultures of Populus alba L. (original cells) require at least 10 M boron for appropriate growth. Using original cells we established a cell line, T-5B, which can grow in a medium containing low levels of boron (5 M). The level of boron localized in the cell walls of T-5B cells was one-half that found in the cell walls of original cells maintained in medium containing 100 M boron, and the level of the rhamnogalacturonan II dimer, cross-linked by a borate ester, also decreased in the former. The sugar composition of whole cell walls of the T-5B cell line was similar that of the original cells, however pectic polysaccharides composed of arabinose or galacturonic acid were easily extracted from T-5B cell walls with 50 mM trans-1,2-cyclohexanediamine-N,N,N ,N -tetraacetic acid. Our results suggest that boron deficiency causes a weakening of the interaction among pectic polysaccharides due to a decrease in boron-rhamnogalacturonanII cross-linkage. |
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Keywords: | Boron deficiency Cell wall Pectic polysaccharide Populus alba L Rhamnogalacturonan II |
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