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Transfer of E. coli gutD gene into maize and regeneration of salt-tolerant transgenic plants |
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| Authors: | LIU YanWANG GuoyingLIU JunjunPENG XuexianXIE YoujuDAI JingruiGUO ShiweiZHANG Fusuo |
| Institution: | (1) College of Plant Science and Technology, China Agricultural University, 100094 Beijing, China;(2) State Key Laboratory for Agrobiotechnology, China Agricultural University, 100094 Beijing, China;(3) Department of Plant Nutrition, China Agricultural University, 100094 Beijing, China;(4) Institute of Microbiology, Chinese Academy of Sciences, 100080 Beijing, China |
| Abstract: | GutD gene, encoding a key enzyme (glucitol-6-phosphate dehydrogenase) of sugar alcohol metabolic pathway inE. coli, was transferred into maize. Results of Southern and Western blotting analysis certified that this gene had integrated and been expressed in transgenic maize plants and their progeny. The synthesis and accumulation of sorbitol were detected in transgenic maize plants and a preliminary nutrient solution culture experiment showed thatgutD transgenic maize plants had an increased tolerance to salt stress compared with nontransgenic ones. Project supported by the National Natural Science Foundation of China (Grant No. 39670413) and “863” State High Technology Development Program. |
| Keywords: | maize transformation gutD gene sorbitol salt tolerance |
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