| Isolation and Structural Analysis of an Acidic Polysaccharide from Astragalus membranaceus （Fisch.） Bunge |
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| 引用本文： | Shun-Chun Wang Jun-Jie Shan Zheng-Tao Wang Zhi-Bi Hu. Isolation and Structural Analysis of an Acidic Polysaccharide from Astragalus membranaceus （Fisch.） Bunge[J]. 植物学报(英文版), 2006, 48(11): 1379-1384. DOI: 10.1111/j.1744-7909.2006.00325.x |
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| 作者姓名： | Shun-Chun Wang Jun-Jie Shan Zheng-Tao Wang Zhi-Bi Hu |
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| 作者单位： | Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 基金项目： | Supported by the Shanghai Leading Academic Discipline Project （Y0301）. |
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| 摘 要： | A new water-soluble hetero-polysaccharlde, APSID3, was obtained from a hot-water extract of the roots of Astragalus membranaceus （Flsch.） Bunge by DEAE-Sepharose Fast Flow and Sephacryl S-300 chromatography. The molecular weight of APSID3 was estimated to be 5.79 × 10^5 Da. Based on a sugar composlUon analysis, methylatlon analysis, partial hydrolysis and 13C nuclear magnetic resonance experimentation, It was concluded that the minimal repeat unit of APSID3 was composed of one terminal arablnose, one 1,5-1Inked arabinose, one 1,3-1Inked rhamnose, one 1,3,4-1Inked rhamnose, five 1,4-1Inked methyl galacturonates and six 1,4-1inked methyl glucuronates.
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| 关 键 词： | 隔离措施 酸性多糖 净化方式 植物 |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2005-11-232006-05-15 |
Isolation and Structural Analysis of an Acidic Polysaccharide from Astragalus membranaceus (Fisch.) Bunge |
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| Shun-Chun Wang,Jun-Jie Shan,Zheng-Tao Wang , Zhi-Bi Hu. Isolation and Structural Analysis of an Acidic Polysaccharide from Astragalus membranaceus (Fisch.) Bunge[J]. Journal of integrative plant biology, 2006, 48(11): 1379-1384. DOI: 10.1111/j.1744-7909.2006.00325.x |
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| Authors: | Shun-Chun Wang Jun-Jie Shan Zheng-Tao Wang Zhi-Bi Hu |
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| Affiliation: | Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine;, Shanghai 201203, China) |
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| Abstract: | A new water‐soluble hetero‐polysaccharide, APSID3, was obtained from a hot‐water extract of the roots of Astragalus membranaceus (Fisch.) Bunge by DEAE‐Sepharose Fast Flow and Sephacryl S‐300 chromatography. The molecular weight of APSID3 was estimated to be 5.79 × 105Da. Based on a sugar composition analysis, methylation analysis, partial hydrolysis and 13C nuclear magnetic resonance experimentation, it was concluded that the minimal repeat unit of APSID3 was composed of one terminal arabinose, one 1,5‐linked arabinose, one 1,3‐linked rhamnose, one 1,3,4‐linked rhamnose, five 1,4‐linked methyl galacturonates and six 1,4‐linked methyl glucuronates. (Managing editor: Wei Wang) |
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| Keywords: | Astragalus membranaceus hetero-polysaccharide isolation and purification structural analysis. |
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