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发育过程中苹果果皮和果肉细胞的超微结构
引用本文:彭宜本 张大鹏. 发育过程中苹果果皮和果肉细胞的超微结构[J]. Acta Botanica Sinica, 2000, 42(8): 794-802
作者姓名:彭宜本 张大鹏
作者单位:中国农业大学果树分子发育生物学实验室!北京100094
基金项目:TheNationalNaturalScienceFoundationofChina ( 3 973 0 3 4 0 ) .
摘    要:用透射电镜对发育过程中苹果(Malus domestica Borkh cv.Red Fuji)果皮和果肉细胞的超微结构进行了观察。结果表明,不同发育期果皮细胞的超微结构发生了变化,其中最引人注目的是,内质网自始至终密布于整个细胞质中,而且大多为槽库膨大的、合成功能旺盛的管状粗面内质网,并分泌出大量的具运输功能的小泡;观察到这些小泡与液泡融合的景象;细胞中也存在活跃的高尔基体。超微结构上的这些现象

关 键 词:苹果 果实 果皮 果肉 超微结构 果实发育

Ultrastructure of Epidermis and Flesh of the Developing Apple Fruit
Abstract:The ultrastructure of the epidermis and flesh of apple ( Malus domestica Borkh cv. Red Fuji) fruit was systematically observed during the fruit development via transmission electron microscopy. The results showed that, in spite of the ultrastructural changes in many aspects of the developing fruit epidermal cells, it remained almost unchanged throughout the whole developmental process that the cytoplasm was filled with numerous endoplasmic reticula (ER). Most of these endoplasmic reticula were tube_like and rough_ER with enlarged cisterna from which many vesicles were produced. Some of the vesicles were shown to merge into vacuole. Some dynamic Golgi bodies were also found. All the ultrastructural characteristics showed that the epidermal cells have the features of excretory cells. The ultrastructure of the fruit flesh cells at the young fruit stage were shown to be metabolically active, characterized by the presence of numerous clustered plasmodesmata, cisterna enlarged_ and rough_ER filling the cytoplasm, plenty of vesicles and Golgi bodies, indicating their dynamic cellular transport function. Some giant_circular rough_ERs were found. All the ultrastructural features at this early developmental stage should be closely associated with the enlargement of the young fruit. At the rapid growing phase of the fruit the main changes were characterized by: the starch grain_filled amyloplasts, furcating of the single orifice of plasmodesmata, and the cytoplasm enrichment of both the Golgi body_formed vesicles and other vesicles. These features correspond well with those of a photoassimilate sink_cell. An ultrastructural degeneration phenomenon was observed at the fruit ripening stage, but the mitochondria and plasmalemma still remained intact, which might be related to the continuous development of fruit quality during the fruit ripening.
Keywords:apple  fruit  epidermis  flesh  ultrastructure  fruit development
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