Effects of prostaglandins and catecholamines on rat spleen adenylate cyclase in vitro |
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Authors: | Thomas J Wincek Floyd W Sweat |
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Institution: | Department of Biological Chemistry, University of Utah College of Medicine, Salt Lake City, Utah 84132 U.S.A. |
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Abstract: | The results reported here show some characteristics of adenylate cyclase (EC 4.6.1.1) derived from homogenates of rat spleen, and describe the in vitro stimulation of this enzyme by prostaglandins, nucleotides, and F− under conditions where cyclic nucleotide degradative pathways are effectively inhibited.Particulate fractions from rat spleen homogenates contain high adenylate cyclase activities, and the highest specific activity is recovered in a particulate fraction prepared by low speed (1200 × g) centrifugation. Activity found in all particulate fractions is stimulated by fluoride, prostaglandins E1 and E2, catecholamines, and purine nucleotides. No stimulation is caused by prostaglandins F1α and F2α. Stimulation by prostaglandin E1 or E2 is augmented by GTP and other purine nucleotides, and stimulation by the combination of GTP and prostaglandin E1 is equal to that caused by optimal fluoride concentrations. Stimulation c caused by L-isoproterenol is additive to that caused by GTP but is not increased by GTP. |
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Keywords: | To whom correspondence should be addressed |
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