Localization of choline acetyltransferase in human brain stem neurons

Berth's method was used to study the cytochemical activity of choline acetyltransferase in truncus cerebri neurons of 6-8 lunar month-old human fetuses. Three types on neurons were diagnosed in the nuclei of the truncus cerebri with regard to cholinacetyltransferase localization: (1) cholinergic cholinoceptive neurons; (2) cholinergic non-cholinoceptive neurons; (3) non-cholinergic cholinoceptive neurons. The distribution of the neurons in 27 nuclei of the truncus cerebri is described.