Regulation of taurine transport in murine macrophages |
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Authors: | Romio L Zegarra-Moran Varesio L Galietta LJV |
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Institution: | (1) Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genova, Italy, IT;(2) Laboratorio di Biologia Molecolare, Istituto Giannina Gaslini, Genova, Italy, IT |
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Abstract: | Summary. We studied the regulation of taurine transport in ANA1 murine macrophage cell line. Taurine uptake was upregulated by hypertonicity
and downregulated by bacterial lypopolysaccharide (LPS) and other stimuli leading to macrophage activation. However combined
stimulation with LPS plus hypertonic shock evoked an increase of taurine uptake that was even higher than with hypertonic
shock alone. Taurine transport was not modified by LPS in GG2EE macrophages derived from C3H/Hej mouse strain, which harbour
a mutated Toll-like receptor 4 (TLR4) and thus are not activated by LPS. The extracellular signal-regulated kinase (ERK) inhibitor
PD98059 abrogates the effect of both LPS and hyperosmotic shock on ANA1 taurine uptake, while the p38 inhibitor SB203580 reduces
the taurine uptake in control conditions and impairs only the response to hypertonicity. These results suggest that the effect
of LPS on taurine transport depends on ERK pathway and can be influenced by environmental conditions.
Received September 1, 2000 Accepted December 6, 2000 |
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Keywords: | : Amino acids Cell volume regulation Hypertonic shock Bacterial lypolysaccharide Organic osmolytes Endotoxin resistance |
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