Production of tetraploid plants of non apomictic citrus genotypes |
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Authors: | Pablo Aleza José Juárez Patrick Ollitrault Luis Navarro |
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Institution: | 1.Centro de Protección Vegetal y Biotecnología,Instituto Valenciano de Investigaciones Agrarias (IVIA),Moncada,Spain;2.UPR amélioration génétique des espèces à multiplication végétative,Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD),Moncada,Spain |
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Abstract: | Ploidy manipulation in Citrus is a major issue of current breeding programs aiming to develop triploid seedless mandarins
to address consumer demands for seedless fruits. The most effective method to obtain triploid hybrids is to pollinate tetraploid
non apomictic cultivars with pollen of diploid varieties. Such non apomictic tetraploid lines are not found in the citrus
germplasm and need to be created. In this work we describe a new methodology based on in vitro shoot-tip grafting combined
with treatment of the micro-grafted shoot-tip with colchicine and oryzalin to achieve chromosome doubling and a dechimerization
procedure assisted by flow cytometry. Stable tetraploid plants of Clemenules, Fina and Marisol clementines and Moncada mandarin
have been obtained directly from shoot tip grafting combined with colchicine and oryzalin treatments or after dechimerization
of mixoploids plants (2x–4x). These stable tetraploid plants have been used in 4x × 2x hybridizations, to recover over 3,250 triploid hybrids in 3 years. |
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