Electrochromatography of amino acid mixtures in fermentation liquors |
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| Authors: | M K El-Marsafy Z ?ehá?ek Abdel-Gawad Zenab |
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| Institution: | 1. Department of Antibiotics and Experimental Mycology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 6
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| Abstract: | The substitution of the phenolic chromatography dimension, by electrophoresis at 500 to 1000 volts, in a veronal buffer, pH 8.6, μ 0.02 followed by triple rechromatography in a completely miscible butanol, acetic acid, water solvent system, leads to clear, easily reproducible electrochromatography patterns. A reliable economical chromatography cabinet is described. The technique allows the analysis of many samples conveniently, and some important characteristics of unidentifiable components can be readily predicted. |
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