In vitro pollination of isolated ovules of Cichorium intybus L. |
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| Authors: | C. I. Castaño M. P. DeProft |
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| Affiliation: | (1) Faculty of Agricultural and Applied Biological Sciences, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001 Heverlee, Belgium e-mail: claracastano@yahoo.com Fax: +32-16-322966, BE |
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| Abstract: | A method for the in vitro pollination of chicory (Cichorium intybus L.) ovules was developed. The purpose was to avoid self-incompatibility after in vitro self-pollination of ovules isolated from flower buds before anthesis and from open flower heads. Seedlings were obtained at a low percentage (0.76%), and the results were explained in terms of pollen viability, pollen germination on the ovule, embryogenesis studies and ploidy analysis. Received: 9 April 1997 / Revision received: 23 July 1997 / Accepted: 6 September 1999 |
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| Keywords: | Chicory In vitro pollination Pollen viability Self-incompatibility |
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