Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (<Emphasis Type="Italic">Acrocomia aculeata</Emphasis> (Jacq.) Lodd. ex Martius) |
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Authors: | Elisa Ferreira Moura Marília Contin Ventrella Sérgio Yoshimitsu Motoike Adauto Quirino de Sá Júnior Mychelle Carvalho Candida Elisa Manfio |
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Institution: | 1.Laboratório de Cultura de Tecidos Vegetais, Departamento de Fitotecnia,Universidade Federal de Vi?osa,Vicosa,Brazil;2.Laboratório de Anatomia Vegetal, Departamento de Biologia Vegetal,Universidade Federal de Vi?osa,Vicosa,Brazil |
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Abstract: | The aim of this paper was to describe the histological events that led to somatic embryogenesis in macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius). Zygotic embryos were inoculated on Y3 medium containing 9 μM 4-amino-3,5,6-trichloropicolonic acid (picloram). Somatic embryos regenerated from nodular callus
on induction medium with activated charcoal under photoperiod or without activated charcoal under dark. Many proembryos originated
from the fundamental meristem after 10–20 days of culture. When transferred to medium containing activated charcoal, under
photoperiod, calli regenerated into somatic embryos of unicellular origin. These embryos had protoderm, plumule and procambial
strands and some of them could germinate. After 30–40 days of culture, meristematic masses grew from procambial cells. The
masses generated nodular callus, and after transfer to medium without activated charcoal, under dark, they generated somatic
embryos of multicellular origin. Those embryos did not regenerate into plants. |
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Keywords: | Oleaginous palm Morphogenesis Plant tissue culture Picloram |
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