Crystallization and preliminary crystallographic data of a Streptomyces scabies extracellular esterase. |
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Authors: | R Green J L Schottel L Swenson Y Wei Z S Derewenda |
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Affiliation: | Department of Biochemistry, University of Alberta, Edmonton, Canada. |
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Abstract: | X-ray quality single crystals of an extracellular esterase from pathogenic Streptomyces scabies were obtained by the hanging drop method. The crystals are monoclinic (space group C2, a = 161.1 A, b = 51.2 A, c = 124.2 A, beta = 100.6 degrees) with two molecules related by a noncrystallographic dyad in the asymmetric unit, with a solvent content of approximately 64%. The diffraction pattern from fresh crystals extends beyond 2 A resolution using sealed tube CuK alpha radiation. The study has been initiated in order to elucidate the mechanism of this unusual non-serine-dependent esterase, and to gain better understanding of the molecular basis of the pathogenesis of the scab disease. |
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