Expression in monkey cells of the missing enzyme in L-ascorbic acid biosynthesis, L-gulono-gamma-lactone oxidase |
| |
| Authors: | K Yagi T Koshizaka M Kito T Ozawa M Nishikimi |
| |
| Institution: | Institute of Applied Biochemistry, Yagi Memorial Park, Japan. |
| |
| Abstract: | L-Gulono-gamma-lactone oxidase (GLO), an enzyme that is missing in scurvy-prone animals, was produced in COS-1 cells by transfection with a minigene constructed from pSVL vector and rat GLO cDNA. A functional GLO protein was expressed, which was indistinguishable from rat GLO in molecular size. The microsomes obtained from the transfected COS-1 cells showed GLO activity comparable to that in rat liver microsomes. |
| |
| Keywords: | |
|
|
