Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars |
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Authors: | Cheng Liu Pete Callow Lisa J Rowland James F Hancock Guo-qing Song |
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Institution: | (1) Plant Biotechnology Resource and Outreach Center, Department of Horticulture, Michigan State University, East Lansing, MI 48824, USA;(2) USDA-ARS, Genetic Improvement of Fruits and Vegetables Lab, Beltsville, MD 20705, USA;(3) Present address: Liaoning Institue of Pomology, 115009 Xiongyue, Liaoning, People’s Republic of China; |
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Abstract: | Protocols were developed to optimize adventitious shoot regeneration from four southern highbush blueberry cultivars. Leaf
explants from 6 week-old shoots of the four cultivars were excised and cultured on woody plant medium each containing thidiazuron
(4.54 or 9.08 μM), zeatin (18.2 μM), or zeatin riboside (5.7 or 11.4 μM) either separately or in combination with α-naphthaleneacetic
acid at 2.69 μM. Optimum medium for shoot regeneration was genotype-dependent. Efficient regeneration was obtained at frequencies
of 88.9% for ‘Jewel’, 87.8% for ‘Emerald’, 53.3% for ‘Jubilee’ and 87.8% for ‘Biloxi’. Leaf explants of newly developed shoots
from the cultures having undergone five subcultures had higher regeneration frequencies than those having undergone two subcultures.
Regenerated shoots, 80–100% for each cultivar, rooted in 8 weeks after transplantation to soil. The regeneration systems described
have potential use in genetic transformation of southern highbush blueberry cultivars. |
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