Application of Spin Trapping to Human Phagocytic Cells: Insight Into Conditions for Formation and Limitation of Hydroxyl Radical |
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Authors: | Myron S Cohen Bradley E Britigan Sovitj Pou Gerald M Rosen |
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Institution: |
a Departments of Medicine, Microbiology and Immunology, University of North Carolina at Chapel Hill., Chapel Hill, NC
b Department of Medicine, University of Iowa College of Medicine and Veterans Administration Medical Center, Iowa City, IA
c Department of Pharmncology and Tooxicology, University of Maryland School of Pharmacy, Baltimore, MD
d Veterans Administration Medical Center, Baltimore, MD |
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Abstract: | In recent years spin trapping techniques have been used extensively to better understand the free radical biology of phagocytic cells. These results demonstrate that spin trapping is of adequate sensitivity to detect superoxide and/or hydroxyl radical generated by these cells, and that spin trapping is capable of measuring phagosomal radicals as well. However. neither neutrophils. monocytes. nor monocyte derived macro-phages generate hydroxyl radical in the absence of exogenous iron. Furthermore. neutrophil lactoferrin and myeloperoxidase limit the magnitude (and in the case of lactoferrin the duration) of hydroxyl radical formed by neutrophils in an iron catalyzed system. Since monocytic phagocytes posxss no lactoferrin, and limited myeloperoxidase, hydroxyl radical may play an important role in the inflammatory behavior of mononuclear phagocytes. |
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Keywords: | Phagocytes Neutrophils Monocytes spin-trapping superoxide hydroxyl radical |
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