数字PCR仪校准方法研究

数字PCR仪是核酸绝对定量的重要仪器，因此确保数字PCR仪检测结果的准确性十分重要。通过对国内市场上数字PCR仪的比较分析，剖析了数字PCR仪的性能指标，对数字PCR仪的校准方法进行了探讨，设定了拷贝数浓度相对示值误差、拷贝数浓度重复性、荧光通道一致性和反应单元个数重复性作为数字PCR仪整机校准的计量技术指标，采用具有溯源性的国家有证标准物质，对方法进行了试验验证。验证结果表明了校准思路和方法的可行性，该方法操作性强，能够满足仪器技术要求以及用户需求, 提高了数字PCR仪检测结果的准确性和可靠性,对进一步拓展和深化数字PCR 技术的应用具有积极的促进作用。

Research on Calibration Method of Digital PCR Instrument

The digital PCR instrument is an important instrument for absolute quantification of nucleic acids, and it is of great significance to ensure the accuracy of the detection results of the digital PCR instrument. Different digital PCR instruments on the domestic market were compared, the performance indexes of the digital PCR instrument were analyzed and the calibration method of the instrument were discussed. Several key measurement technical indicators of the digital PCR machine calibration were set, including the copy number concentration relative indication error, copy number concentration repeatability, fluorescence channel consistency and the number of reaction unit repeatability. The method was tested and verified by utilizing traceable national certified reference materials. The verification results showed the feasibility of the calibration method. The method has strong operability, can meet the technical requirements of the instrument and the needs of users, improve the accuracy and reliability of the detection results of the digital PCR instrument, and has a positive role in further expanding and deepening the application of the digital PCR technology.