| Abstract: | Internal reference gene refers to a conserved DNA sequence which can distinguish species specificity, has a single or constant low copy number, and low variation. The internal reference genes of designated crop species are used as the main genetic markers to identify other species in many fields, such as transgenic component detection, species and product discrimination, etc. Especially in the quantitative and qualitative detection of genetically modified products, they can be used to detect species sources and transgenic content in samples, which is of great significance to the quantitative and qualitative detection of genetically modified products. At present, many kinds of internal reference genes of crops have been developed, among which the number of internal reference genes developed for maize, rape and rice is relatively large, but there is still a lack of more thorough comparative research, which makes it difficult to select and apply them. Based on this, according to the existing research results at home and abroad, the research progress of internal reference genes of six crops (including corn, soybean, rape, cotton, rice and wheat) with great demand for detection and identification was summarized, and the internal reference genes of common crops were systematically compared and studied, in order to provide technical support for plant-derived and transgenic components and related detection and identification. |
