Role of cysteine sulfhydryl groups in activation of adenylyl cyclase signaling system by biogenic amines in molluscan and rat tissues |
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Authors: | A O Shpakov S A Plesneva L A Kuznetsova |
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Institution: | (1) Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia |
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Abstract: | It has been shown that in smooth muscles of the freshwater bivalve molluscAnodonta cygnea as well as in skeletal muscles and brain striatum of rats a blocker of SH-groups,para-chlormercury benzoate (ChMB), and an alkylating agent,N-ethylmaleimide, inhibit both the basal adenylyl cyclase (AC) activity and the activity of the enzyme stimulated by non-hormonal
agents (NaF, GppNH]p) and by hormonal agents such as serotonin (mollusc muscles, rat brain) or isoproterenol (rat muscles
and rat brain). The inhibitory effects of ChMB andN-ethylmaleimide on AC are partly eliminated by an SH-group containing reagent, β-mercaptoethanol (ME, 5 mM). Restoration of
the basal and of the stimulated enzyme activity inhibited by ME is better in the case of the ChMB than of theN-ethylmaleimide action. It has also been found that ME stimulates both the basal and the stimulated by non-hormonal agents
AC activity. In the presence of ME the hormonal stimulating effects on the enzyme are also preserved, except for the effect
of isoproterenol on AC in rat skeletal muscles; this effect is inhibited by ME. Potentiation of the stimulating effect of
the hormones on AC by GppNH]p is only preserved in the molluscan smooth muscles (the effect of serotonin—90%). The data obtained
indicate that cysteine sulfhydryl groups play a key role in hormonal regulation of the functional activity of the components
of the hormone-sensitive adenylyl cyclase signaling system. |
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