Generation of transgenic dogs that conditionally express green fluorescent protein |
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Authors: | Kim Min Jung Oh Hyun Ju Park Jung Eun Kim Geon A Hong So Gun Jang Goo Kwon Mo Sun Koo Bon Chul Kim Teoan Kang Sung Keun Ra Jeong Chan Ko Chemyong Lee Byeong Chun |
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Institution: | Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 151-744, Korea. |
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Abstract: | We report the creation of a transgenic dog that conditionally expresses eGFP (enhanced green fluorescent protein) under the regulation of doxycycline. Briefly, fetal fibroblasts infected with a Tet-on eGFP vector were used for somatic cell nuclear transfer. Subsequently reconstructed oocytes were transferred to recipients. Three clones having transgenes were born and one dog was alive. The dog showed all features of inducible expression of eGFP upon doxycycline administration, and successful breeding resulted in eGFP-positive puppies, confirming stable insertion of the transgene into the genome. This inducible dog model will be useful for a variety of medical research studies. |
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Keywords: | dog transgenic somatic cell nuclear transfer tetracycline vector |
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