| 微生物气溶胶检测技术的研究进展 |
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| 引用本文： | 卢维来,许添顺,汤晖,蒋靖坤,付钰. 微生物气溶胶检测技术的研究进展[J]. 微生物学报, 2022, 62(4): 1345-1361 |
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| 作者姓名： | 卢维来 许添顺 汤晖 蒋靖坤 付钰 |
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| 作者单位： | 中国科学院微生物研究所, 微生物资源前期开发国家重点实验室, 北京 100101;中国科学院微生物研究所, 微生物资源前期开发国家重点实验室, 北京 100101;河北大学生命科学学院, 河北 保定 071002;清华大学环境学院, 北京 100084;中国科学院微生物研究所, 微生物资源前期开发国家重点实验室, 北京 100101;中国科学院大学存济医学院, 北京 100049 |
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| 基金项目： | 国家自然科学基金(52091541,91751103);中国科学院率先杯项目(GQRC-19-18);国家重点研发计划(2019YFA905500);中国科学院"科学"号高端用户项目(KEXUE2019GZ05) |
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| 摘 要： | 生物气溶胶中微生物的种类复杂多样,其采集和鉴定是全面掌握微生物气溶胶生物特性的关键,对防控气溶胶病原体传播十分重要.本文简要综述了微生物气溶胶的生物特性和潜在危害性,介绍了微生物气溶胶的一般采集方法,采集器采集生物气溶胶的基本原理、特点和优缺点.将有助于研究人员根据实验目的在采集低浓度生物气溶胶时选择合适的采样器.本文...
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| 关 键 词： | 生物气溶胶 采集方法 鉴定方法 |
| 收稿时间： | 2021-08-31 |
| 修稿时间： | 2021-12-09 |
Technologies for microbial aerosol sampling and identification:a review and current perspective |
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| LU Weilai,XU Tianshun,TANG Hui,JIANG Jingkun,FU Yu. Technologies for microbial aerosol sampling and identification:a review and current perspective[J]. Acta microbiologica Sinica, 2022, 62(4): 1345-1361 |
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| Authors: | LU Weilai XU Tianshun TANG Hui JIANG Jingkun FU Yu |
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| Affiliation: | State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;School of Life Sciences, Hebei University, Baoding 071002, Hebei, China;School of Environment, Tsinghua University, Beijing 100084, China; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | The bioaerosol harbors diverse microorganisms.The sampling of microbial aerosols and the identification of microorganisms in aerosols are the key to understanding the biological characteristics of microbial aerosols,which is essential for preventing bioaerosol-mediated pathogen transmission.This article briefly reviewed the biological characteristics and potential hazards of microbial aerosols and introduced the general methods for microbial aerosol sampling with respect to their performance,operating mechanisms,advantages,and limitations,in an aim to assist the design of best practices for bioaerosol sampling.Three major (culture-dependent,culture-independent,and single-cell) techniques for the identification of bacteria,fungi,and viruses in bioaerosols were expounded.Finally,the future techniques for microbial aerosol collection and microbial identification were prospected.The cross-disciplinary interaction will facilitate the development of novel methods for bioaerosol sampling and detection.With this review,we hope to provide a theoretical basis for the development of sampling methods and comprehensive characterization of bioaerosols. |
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| Keywords: | bioaerosol sampling method identification method |
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